Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5. Lane 1: TRIM32 transfected lysatE (72 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of monoclonal antibody to TRIM32 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 over-expressed 293 cell line, cotransfected with TRIM32 Validated Chimera RNAi or non-transfected control. Blot probed with H00022954-M09. GAPDH (36.1 kDa) used as loading control.
Sandwich ELISA: TRIM32 Antibody (2E5) [H00022954-M09] - Detection limit for recombinant GST tagged TRIM32 is approximately 0.3ng/ml as a capture antibody.