TRIM32

Gene Symbol TRIM32
Entrez Gene 22954
Alt Symbol BBS11, HT2A, LGMD2H, TATIP
Species Human
Gene Type protein-coding
Description tripartite motif containing 32
Other Description 72 kDa Tat-interacting protein|E3 ubiquitin-protein ligase TRIM32|TAT-interactive protein, 72-KD|tripartite motif-containing 32|tripartite motif-containing protein 32|zinc finger protein HT2A|zinc-finger protein HT2A
Swissprots Q13049 Q9NQP8
Accessions CAX86996 CAY39310 EAW87446 EAW87447 Q13049 AK314264 AX775779 CAE11589 BC003154 AAH03154 BI544919 BX091494 DN991209 DQ893081 ABM84007 DQ896342 ABM87341 U18543 AAA86474 XM_005251813 XP_005251870 XM_011518396 XP_011516698 XM_011518397 XP_011516699 XM_011518398 XP_011516700 NM_001099679 NP_001093149 NM_012210 NP_036342
Function Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. {ECO:0000269|PubMed:19349376, ECO:0000269|PubMed:22500027}.
Subcellular Location Cytoplasm. Note=Localized in cytoplasmic bodies, often located around the nucleus.
Tissue Specificity Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle. {ECO:0000269|PubMed:11822024}.
Top Pathways Ubiquitin mediated proteolysis

TRIM32 (H-88) - sc-99011 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TRIM32 (H-88): sc-99011. Western blot analysis of TRIM32 expression in HeLa (A) and A-431 (B) whole cell lysates.

TRIM32 (N-12) - sc-49265 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TRIM32 (T-20) - sc-49266 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TRIM32 (8H8) - sc-135588 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
TRIM32 (8H8): sc-135588. Western blot analysis of TRIM32 expression in human TRIM32 transfected (A) and non-transfected (B) 293T whole cell lysates.

TRIM32 (G-25) - sc-134120 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
TRIM32 (G-25): sc-134120. Western blot analysis of human TRIM32 transfected 293T whole cell lysate.

Anti-TRIM32 antibody - ab172411 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-TRIM32 antibody (ab172411) at 1 µg/mlLane 1 : TRIM32 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

Anti-TRIM32 antibody - ab56907 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56907 TRIM32 antibody at 1ug/ml.

Anti-TRIM32 antibody [EP6355] - ab131223 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP6355
Anti-TRIM32 antibody [EP6355] (ab131223) at 1/1000 dilution + Human fetal muscle lysate at 10 µgSecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-HT2A / TRIM32 Antibody (N-Terminus) - LS-C31362 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
A: Marker, B: Jurkat Cell Lysate

Anti-HT2A / TRIM32 Antibody (Internal, clone EP6355) - LS-C154529 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP6355
Western blot analysis on fetal muscle lysate using anti-TRIM32 antibody.

Anti-HT2A / TRIM32 Antibody (aa559-608) - LS-C80244 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
TRIM32 antibody LS-C80244 was used in IHC to stain formalin-fixed, paraffin-embedded human muscle.

Anti-HT2A / TRIM32 Antibody (aa1-289) IHC-plus™ - LS-B10897 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-HT2A / TRIM32 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10897 concentration 7.5 ug/ml.

Anti-HT2A / TRIM32 Antibody (aa169-198) - LS-C160187 from LifeSpan Bioscience

Prices $250.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TRIM32 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This demonstrates the use of TRIM32 Antibody (N-term) for immunohistochemistry.

Anti-HT2A / TRIM32 Antibody (aa549-598) IHC-plus™ - LS-B1105 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TRIM32 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1105 concentration 5 ug/ml.

TRIM32 Antibody [Unconjugated] - AF6515 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal

TRIM32 Antibody - NBP1-33737 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: TRIM32 Antibody [NBP1-33737] - A. 30 ug 293T whole cell lysate/extract 7.5 % SDS-PAGETRIM32 antibody dilution: 1:1000

TRIM32 Antibody (717801) - MAB6515 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 717801
Western blot shows lysates of A172 human glioblastoma cell line, DU145 human prostate carcinoma cell line, and PC‑3 human prostate cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human TRIM32 Monoclonal Antibody (Catalog # MAB6515) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRIM32 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TRIM32 Antibody - NBP2-13484 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: TRIM32 Antibody [NBP2-13484] - Staining of human cell line A-431 shows positivity in cytoskeleton (intermediate filaments).

TRIM32 Antibody (2E5) - H00022954-M09 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E5
Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5. Lane 1: TRIM32 transfected lysatE (72 KDa). Lane 2: Non-transfected lysate.

TRIM32 Antibody - H00022954-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

TRIM32 Antibody - H00022954-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

TRIM32 Antibody - H00022954-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

TRIM32/BBS11 Polyclonal Antibody - bs-0964R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

TRIM32/BBS11 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-0964R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

TRIM32/BBS11 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-0964R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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