Western Blot: Exosome Component 9 Antibody [NBP2-22242] - Whole cell lysate (15 and 50 ug) from mouse NIH3T3 cells. NBP2-22242 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Western Blot: Exosome Component 9 Antibody [NBP2-22242] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22242 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: Exosome Component 9 Antibody [NBP2-22242] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22242 used for IP at 6 ug/mg lysate. EXOSC9 was also immunoprecipitated by rabbit anti-EXOSC9 antibody. For blotting immunoprecipitated EXOSC9, NBP2-22242 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.