Western Blot: UPF2 Antibody [NBP2-22281] - Whole cell lysate from mouse NIH3T3 (50 ug), TCMK-1 (50 ug), and 4T1 (50 ug) cells. NBP2-22281 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: UPF2 Antibody [NBP2-22281] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22281 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: UPF2 Antibody [NBP2-22281] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22281 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated RENT2, NBP2-22281 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.