UPF2

Gene Symbol UPF2
Entrez Gene 26019
Alt Symbol HUPF2, RENT2, smg-3
Species Human
Gene Type protein-coding
Description UPF2 regulator of nonsense transcripts homolog (yeast)
Other Description nonsense mRNA reducing factor 2|regulator of nonsense transcripts 2|smg-3 homolog, nonsense mediated mRNA decay factor|up-frameshift suppressor 2 homolog|yeast Upf2p homolog
Swissprots Q5W0J4 Q14BM1 Q8N8U1 Q9P2D9 A6NLJ5 Q9HAU5 Q9Y4M9 D3DRS0 Q9NWL1 Q9H1J2
Accessions CCO13846 EAW86328 EAW86329 EAW86330 EAW86331 EAW86332 EAW86333 Q9HAU5 AB037829 BAA92646 AF301013 AAG33225 AF318574 AAG60689 AK000764 BAA91369 AK074375 BAB85064 AK096191 BAC04721 AK124930 AK295209 BAG58205 AK310925 AL080198 CAB45771 AW444636 AY013249 AAG48509 BC054011 BC090919 AAH90919 BC114964 AAI14965 BC115737 AAI15738 BX647325 DA188353 DB065422 XM_011519447 XP_011517749 XM_011519448 XP_011517750 XM_011519449 XP_011517751 XR_930489 NM_015542 NP_056357 NM_080599 NP_542166
Function Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA. {ECO:0000269|PubMed:11163187, ECO:0000269|PubMed:16209946, ECO:0000269|PubMed:18066079}.
Subcellular Location Cytoplasm, perinuclear region {ECO:0000269|PubMed:11073994, ECO:0000269|PubMed:11113196, ECO:0000269|PubMed:11163187}.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:11073994}.
Top Pathways RNA transport, mRNA surveillance pathway

Upf2 (D3B10) Rabbit mAb - 11875 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3B10
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human Upf2 (hUpf2-Myc; +), using Upf2 (D3B10) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457.

Upf2 (D4A7) Rabbit mAb - 8092 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D4A7

Rent2 (G-9) - sc-398812 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rent2 (G-9): sc-398812. Western blot analysis of Rent2 expression in Y79 (A), Jurkat (B), Ramos (C) and MCF7 (D) whole cell lysates.

Rent2 (G-10) - sc-374230 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rent2 (G-10): sc-374230. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Rent2 (H-4) - sc-398424 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rent2 (H-4): sc-398424. Western blot analysis of Rent2 expression in Y79 (A), Jurkat (B), Ramos (C), MCF7 (D) and Hep G2 (E) whole cell lysates.

Rent2 (C-18) - sc-20227 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rent2 (C-18): sc-20227. Western blot analysis of Rent2 expression in Y79 whole cell lysate.

Rent2 (H-300) - sc-48801 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rent2 (H-300): sc-48801. Western blot analysis of Rent2 expression in TK-1 (A), SUP-T1 (B) and GA-10 (C) whole cell lysates.

Rent2 (N-16) - sc-20225 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rent2 (N-16): sc-20225. Western blot analysis of Rent2 expression in Ramos (A) and Raji (B) whole cell lysates.

Anti-hUPF2/RENT2 antibody - ab28712 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-hUPF2/RENT2 antibody (ab28712) at 1/250 dilutionLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human hUPF2/RENT2 peptide (ab32332) at 1 µg/mlLysates/proteins at 15 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-hUPF2/RENT2 antibody - C-terminal - ab153830 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-hUPF2/RENT2 antibody - C-terminal (ab153830) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

Anti-HUPF2 / UPF2 Antibody (aa1250-1262) - LS-C55380 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-HUPF2 / UPF2 Antibody (aa630-656, APC) - LS-C218361 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUPF2 / UPF2 Antibody (aa630-656, AP) - LS-C218362 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUPF2 / UPF2 Antibody (aa630-656, FITC) - LS-C218364 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUPF2 / UPF2 Antibody (aa630-656, Biotin) - LS-C218363 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUPF2 / UPF2 Antibody (aa630-656, Azide-free, HRP) - LS-C218365 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUPF2 / UPF2 Antibody (aa630-656, PE) - LS-C218367 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUPF2 / UPF2 Antibody (aa633-660) - LS-C326206 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUPF2 / UPF2 Antibody (aa51-100) - LS-C116856 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
UPF2 antibody LS-C116856 Western blot of HepG2 cell lysate.

Anti-HUPF2 / UPF2 Antibody (aa630-656) - LS-C160443 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
UPF2 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The UPF2 antibody detected the UPF2 protein (arrow).

Anti-HUPF2 / UPF2 Antibody - LS-C346222 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot blot of extracts of various cell lines, using UPF2 antibody.

Anti-HUPF2 / UPF2 Antibody (aa1-50) - LS-C290577 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RENT2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RENT2 antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated RENT2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

UPF2 Antibody - NBP2-20813 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UPF2 Antibody [NBP2-20813] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:1000.

UPF2 Antibody - NBP2-22281 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UPF2 Antibody [NBP2-22281] - Whole cell lysate from mouse NIH3T3 (50 ug), TCMK-1 (50 ug), and 4T1 (50 ug) cells. NBP2-22281 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

UPF2 Antibody - NBP1-80460 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UPF2 Antibody [NBP1-80460] - HepG2 cell lysate, concentration 0.2-1 ug/ml.
Search more