Western Blot: PHAX Antibody [NBP2-22268] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. NBP2-22268 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: PHAX Antibody [NBP2-22268] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody NBP2-22268 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: PHAX Antibody [NBP2-22268] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22268 used for IP at 6 ug/mg lysate. PHAX was also immunoprecipitated by rabbit anti-PHAX antibody. For blotting immunoprecipitated PHAX, NBP2-22268 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.