Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb
| Name | Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 2717 |
| Prices | $109.00, $287.00 |
| Sizes | 20 µl (2 western blots), 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | 65E4 |
| Applications | WB ICC/IF FC |
| Species Reactivities | Human, Mouse, Rat, Hamster, Chicken, Bovine, Dog, Pig, Horse |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. |
| Description | Rabbit Monoclonal |
| Gene | SYK |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from Jurkat cells treated with hydrogen peroxide (2mM for 2 minutes) or with lambda phosphatase and extracts from Ramos cells treated with anti-human IgM (12 micrograms/ml for 2 minutes) using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb.
Confocal immunofluorescent analysis of Ramos cells, human IgM treated (left) or untreated (right), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.
Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM (green), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.