SYK

Gene Symbol SYK
Entrez Gene 6850
Alt Symbol p72-Syk
Species Human
Gene Type protein-coding
Description spleen tyrosine kinase
Other Description tyrosine-protein kinase SYK
Swissprots P43405
Accessions EAW62782 EAW62783 EAW62784 EAW62785 EAW62786 P43405 AJ420449 AK057772 AK075020 BAG52053 AK094808 AK290927 BAF83616 AL832053 BC001645 AAH01645 BC002962 AAH02962 BC011399 AAH11399 BG754228 BM019270 BU429809 BX647192 DA587403 DA948098 DB458719 FN391891 CAY55929 FN391892 CAY55930 FN391893 CAY55931 L28824 AAA36526 S80267 AAB35601 W33104 X73568 CAA51970 Z29630 CAA82737 XM_005252147 XP_005252204 XM_011518946 XP_011517248 XM_011518947 XP_011517249 XR_929837 NM_001135052 NP_001128524 NM_001174167 NP_001167638 NM_001174168 NP_001167639 NM_003177 NP_003168
Function Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine- phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR
Subcellular Location Cell membrane {ECO:0000305}. Cytoplasm, cytosol {ECO:0000305}.
Tissue Specificity Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells. {ECO:0000269|PubMed:8163536}.
Top Pathways Tuberculosis, Epstein-Barr virus infection, PI3K-Akt signaling pathway, Viral carcinogenesis, Natural killer cell mediated cytotoxicity

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 4349 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C87C1
Confocal immunofluorescent analysis of Ramos cells, untreated (left) or treated with IgM (12 ug/ml, 2 min) (right), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green). Blue Pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (PE Conjugate) - 6485 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C87C1
Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM and hydrogen peroxide (green), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (PE Conjugate).

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Pacific Blue™ Conjugate) - 12967 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C87C1
Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM and hydrogen peroxide (green), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Pacific Blue™ Conjugate).

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Biotinylated) - 5514 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C87C1
Western blot analysis of extracts from Ramos cells, untreated (-) or anti-IgM (10 μg/ml, 30 min) and hydrogen peroxide (H 2 O 2 ) (3.3mM, 25 min) treated (+), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Biotinylated). The western blot was developed using Streptavidin-HRP #3999.

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 12081 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C87C1
Confocal immunofluorescent analysis of Ramos cells untreated (left), or treated with anti-IgM (right), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

Phospho-Syk (Tyr525/526) Antibody - 2711 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM (12ug/ml, 2 minutes) and Jurkat cells treated with hydrogen peroxide (2mM, 2 minutes) using Phospho-Syk (Tyr525/526) Antibody.

Phospho-Syk (Tyr323) Antibody - 2715 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells expressing recombinant wild-type or mutant Syk proteins, cotransfected with CD8, using Phospho-Syk (Tyr323) Antibody (upper) or Syk Antibody #2712 (lower). (Provided by Dr. Alagarsamy L. Reddi, laboratory of Dr. Hamid Band, Harvard University, Massachusetts.)

Phospho-Syk (Ser297) Antibody - 14140 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from serum-starved SR cells, untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-Syk (Ser297) Antibody (upper) and Syk (D3Z1E) XP ® Rabbit mAb #13198 (lower).

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb - 2710 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C87C1
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-IgM, using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (upper) or Syk Antibody #2712 (lower).

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody - 2701 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat cells, starved for 16 hours, and treated with 2 mM H2O2 or with calf intestinal alkaline phosphatase (CIP), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (upper) or control Zap-70 Antibody #2702 (lower).

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb - 2717 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 65E4
Western blot analysis of extracts from Jurkat cells treated with hydrogen peroxide (2mM for 2 minutes) or with lambda phosphatase and extracts from Ramos cells treated with anti-human IgM (12 micrograms/ml for 2 minutes) using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb.

Syk (D3Z1E) XP ® Rabbit mAb - 13198 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3Z1E
Western blot analysis of extracts from various cell lines using Syk (D3Z1E) XP ® Rabbit mAb.

Syk (D1I5Q) Rabbit mAb - 12358 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1I5Q
Western blot analysis of extracts from various cell lines using Syk (D1I5Q) Rabbit mAb.

Syk Antibody - 2712 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat (lane 1) and THP1 (lane 2) cells, and untransfected or Syk cDNA-transfected DT40 cells, treated as indicated (lanes 3-5), using Syk Antibody.

Syk (LR) - sc-573 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Syk (LR): sc-573. Western blot analysis of Syk expression in BJAB (A) and Ramos (B) whole cell lysates.

Syk (D-3) - sc-166226 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Syk (D-3): sc-166226. Western blot analysis of Syk expression in non-transfected: sc-110760 (A) and human Syk transfected: sc-111124 (B) 293 whole cell lysates.

Syk (G-2) - sc-28337 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Syk (G-2): sc-28337. Western blot analysis of Syk expression in non-transfected: sc-110760 (A) and human Syk transfected: sc-111124 (B) whole cell lysates.

Syk (N-19) - sc-1077 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Syk (N-19): sc-1077. Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic staining.

Syk (C-20) - sc-929 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Syk (C-20): sc-929. Western blot analysis of Syk expression in BJAB (A), Ramos (B), NAMALWA (C) and RAW 264.7 (D) whole cell lysates.

Syk (4D10) - sc-1240 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Syk (4D10): sc-1240. Western blot analysis of Syk expression in BJAB whole cell lysate.

Syk (0.T.142) - sc-73086 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Syk (0.T.142): sc-73086. Western blot analysis of Syk expression in non-transfected: sc-110760 (A) and human Syk transfected: sc-111124 (B) 293 whole cell lysates.

Syk (SYK-01) - sc-51703 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Syk (SYK-01): sc-51703. Western blot analysis of Syk expression in non-transfected: sc-110760 (A) and human Syk transfected: sc-111124 (B) 293 whole cell lysates.

Syk (6D784) - sc-73088 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Syk (6D784): sc-73088. Western blot analysis of Syk expression in non-transfected: sc-110760 (A) and human Syk transfected: sc-111124 (B) 293 whole cell lysates.

Syk (6A464) - sc-73089 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Syk (6A464): sc-73089. Western blot analysis of Syk expression in non-transfected: sc-110760 (A) and human Syk transfected: sc-159001 (B) 293 whole cell lysates.

p-Syk (Tyr 323) - sc-293118 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Syk (Tyr 323): sc-293118. Western blot analysis of Syk phosphorylation expression in 3 minute H2O2 treated (A) and 15 minute H2O2 treated (B) JK cell extracts.
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