![Western Blot: CLCN4 Antibody [NBP2-30066] - Chicken brain tissue incubated with CLC4 Antibody (C-term) (NBP2-30066). Data courtesy of Emily McMains, Louisiana State University.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/14850_CLCN4-Antibody-Western-Blot-NBP2-30066-img0001.jpg)
Western Blot: CLCN4 Antibody [NBP2-30066] - Chicken brain tissue incubated with CLC4 Antibody (C-term) (NBP2-30066). Data courtesy of Emily McMains, Louisiana State University.
![Immunocytochemistry/Immunofluorescence: CLCN4 Antibody [NBP2-30066] - Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody (Cat.# AP6329f) for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/14851_CLCN4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30066-img0003.jpg)
Immunocytochemistry/Immunofluorescence: CLCN4 Antibody [NBP2-30066] - Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody (Cat.# AP6329f) for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour.
![Immunocytochemistry/Immunofluorescence: CLCN4 Antibody [NBP2-30066] - Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) (NBP2-30066). Data courtesy of Emily McMains, Louisiana State University.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/14852_CLCN4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30066-img0002.jpg)
Immunocytochemistry/Immunofluorescence: CLCN4 Antibody [NBP2-30066] - Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) (NBP2-30066). Data courtesy of Emily McMains, Louisiana State University.