![Immunocytochemistry/Immunofluorescence: CNOT7 Antibody [NBP2-29987] - Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term)(NBP2-29987).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25, 1 h at 37). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/14777_CNOT7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29987-img0003.jpg)
Immunocytochemistry/Immunofluorescence: CNOT7 Antibody [NBP2-29987] - Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term)(NBP2-29987).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25, 1 h at 37). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
![Immunohistochemistry: CNOT7 Antibody [NBP2-29987] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/14778_CNOT7-Antibody-Immunohistochemistry-NBP2-29987-img0002.jpg)
Immunohistochemistry: CNOT7 Antibody [NBP2-29987] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
![Western Blot: CNOT7 Antibody [NBP2-29987] - Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/14776_CNOT7-Antibody-Western-Blot-NBP2-29987-img0001.jpg)
Western Blot: CNOT7 Antibody [NBP2-29987] - Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).