Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP2-29936] - Antibody (Center) at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry-Paraffin: Kynurenine 3-Monooxygenase/KMO Antibody [NBP2-29936] - Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with KMO Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry: Kynurenine 3-Monooxygenase/KMO Antibody [NBP2-29936] - Flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP2-29936] - Western blot analysis of KMO Antibody (Center) (NBP2-29936) in CEM cell line lysates (35ug/lane). KMO (arrow) was detected using the purified Pab.