Western Blot: IL1F6 Antibody (1A4) [NBP2-02758] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
Immunocytochemistry/Immunofluorescence: IL1F6 Antibody (1A4) [NBP2-02758] Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6.
Flow Cytometry: IL1F6 Antibody (1A4) [NBP2-02758] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IL1F6 antibody, and then analyzed by flow cytometry.