Western Blot: ACD Antibody (1A2) [NBP2-01851] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACD.
Immunocytochemistry/Immunofluorescence: ACD Antibody (1A2) [NBP2-01851] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACD.
Flow Cytometry: ACD Antibody (1A2) [NBP2-01851] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACD antibody, and then analyzed by flow cytometry.