Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 expression in transfected 293T cell line by UBR2 monoclonal antibody (M01), clone 4G4.Lane 1: UBR2 transfected lysate(50 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of monoclonal antibody to UBR2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 over-expressed 293 cell line, cotransfected with UBR2 Validated Chimera RNAi ( Cat # H00023304-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UBR2 monoclonal antibody (M01), clone 4G4 (Cat # H00023304-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: UBR2 Antibody (4G4) [H00023304-M01] - Detection limit for recombinant GST tagged UBR2 is approximately 0.3ng/ml as a capture antibody.