Western Blot: HMGCS1 Antibody [NBP2-36554] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGCS1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: HMGCS1 Antibody [NBP2-36554] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated HMGCS1, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.