Western Blot: PRPF8 Antibody [NBP2-22274] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (5 ug) cells. NBP2-22274 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: PRPF8 Antibody [NBP2-22274] - Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- PRPF8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: PRPF8 Antibody [NBP2-22274] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody NBP2-22274 used for IP at 6 ug/mg lysate. PRPF8 was also immunoprecipitated by rabbit anti-PRPF8 antibodies NBP2-22272 and NBP2-22273 For blotting immunoprecipitated PRPF8, NBP2-22274 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.