Western Blot: PHLPP Antibody [NBP2-22317] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. NBP2-22317 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: PHLPP Antibody [NBP2-22317] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: NBP2-22317 used for IP at 6 ug/mg lysate. PHLPP was also immunoprecipitated by rabbit anti-PHLPP antibody NB100-1811. For blotting immunoprecipitated PHLPP, NBP2-22317 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.