PHLPP1

Gene Symbol PHLPP1
Entrez Gene 23239
Alt Symbol PHLPP, PLEKHE1, SCOP
Species Human
Gene Type protein-coding
Description PH domain and leucine rich repeat protein phosphatase 1
Other Description PH domain leucine-rich repeat-containing protein phosphatase 1|PH domain-containing family E member 1|SCN circadian oscillatory protein|pleckstrin homology domain containing, family E (with leucine rich repeats) member 1|suprachiasmatic nucleus circadian oscillatory protein
Swissprots Q96FK2 A1A4F5 Q86TN6 O60346 Q641Q7 Q6PJI6 Q9NUY1 Q6P4C4
Accessions EAW63135 O60346 AB011178 BAA25532 AF130097 AAG35522 AK001924 BAA91980 AK124714 AL079409 BC010706 AAH10706 BC014927 AAH14927 BC047653 AAH47653 BC063519 AAH63519 BC082244 AAH82244 BC126277 AAI26278 BG110729 XM_011525886 XP_011524188 NM_194449 NP_919431
Function Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser- 657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts. {ECO:0000269|PubMed:15808505, ECO:0000269|PubMed:17386267, ECO:0000269|PubMed:18162466, ECO:0000269|PubMed:19079341}.
Subcellular Location Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Note=In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells.
Tissue Specificity In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues. {ECO:0000269|PubMed:15808505, ECO:0000269|PubMed:19079341}.
Top Pathways PI3K-Akt signaling pathway

SCOP (H-3) - sc-390129 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SCOP (H-3): sc-390129. Western blot analysis of SCOP expression in Y79 nuclear extract.

SCOP (C-12) - sc-46450 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SCOP (K-20) - sc-46451 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SCOP (K-20): sc-46451. Western blot analysis of SCOP expression in Y79 nuclear extract.

SCOP (H-60) - sc-67069 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SCOP (H-60): sc-67069. Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells and endothelial cells.

SCOP (N-12) - sc-46452 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SCOP (N-13) - sc-46453 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PHLPP antibody - ab71972 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PHLPP antibody (ab71972) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µg

Anti-PHLPP antibody - ab181338 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemical analysis of SW480 cells labeling PHLPP with ab181338 at 2.5μg/ml.

Anti-PHLPP antibody - ab135957 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PHLPP antibody (ab135957) at 1/500 dilution + Y79 cell extract at 30 µg

Anti-PHLPP antibody - ab71971 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PHLPP antibody - Aminoterminal end - ab71277 from Abcam

Prices $304.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PHLPP antibody - Aminoterminal end (ab71277) at 1/60 dilution + K562 cell line lysate at 35 µg

Anti-SCOP / PHLPP Antibody (aa1050-1100) - LS-C90128 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SCOP / PHLPP Antibody (C-Terminus) - LS-C126131 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SCOP / PHLPP Antibody (C-Terminus) - LS-C90129 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SCOP / PHLPP Antibody (C-Terminus) - LS-C287531 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SCOP / PHLPP Antibody - LS-C342160 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SCOP / PHLPP Antibody (aa531-560) - LS-C97458 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of anti-PHLPP1 Antibody in K562 cell line lysates (35 ug/lane). PHLPP1 (arrow) was detected using the purified antibody.

Anti-SCOP / PHLPP Antibody - LS-C146688 from LifeSpan Bioscience

Prices $325.00
Sizes 1 ea
Host Rabbit
Clonality Polyclonal
Western blot of SCOP / PHLPP antibody LS-C146688.

Anti-SCOP / PHLPP Antibody (aa1194-1243) - LS-C199994 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from Y79 cells, using SCOP antibody.

Anti-SCOP / PHLPP Antibody (aa1175-1225) - LS-C290673 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse PHLPP by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-PHLPP antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-SCOP / PHLPP Antibody (aa1050-1100) IHC-plus™ - LS-B11305 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-SCOP / PHLPP antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11305 concentration 5 ug/ml.

Anti-SCOP / PHLPP Antibody (N-Terminus) IHC-plus™ - LS-B8860 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SCOP / PHLPP antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PHLPP Antibody - NB100-1810 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: PHLPP Antibody [NB100-1810] - Detection of Human PHLPP on HeLa whole cell lysate using NB100-1810 and NB100-1811. Immunoprecipitated PHLPP blotted with NB100-1810.

PHLPP Antibody - NB100-1811 from Novus Biologicals

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PHLPP Antibody [NB100-1811] - Detection of Human and Mouse PHLPP on HeLa whole cell lysate using NB100-1811. PHLPP was also immunoprecipitated using rabbit anti-PHLPP antibody NB100-1810.

PHLPP Antibody - NBP2-22317 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: PHLPP Antibody [NBP2-22317] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. NBP2-22317 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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