Western Blot: DLST Antibody [NBP2-32204] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-DLST antibody NBP2-32204 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: DLST Antibody [NBP2-32204] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- DLST used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunohistochemistry: DLST Antibody [NBP2-32204] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- DLST used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: DLST Antibody [NBP2-32204] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-DLST antibody NBP2-32204 used for IP at 6 ug per reaction. For blotting immunoprecipitated DLST, NBP2-32204 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.