DLST

Gene Symbol DLST
Entrez Gene 1743
Alt Symbol DLTS
Species Human
Gene Type protein-coding
Description dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
Other Description E2K|OGDC-E2|dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
Swissprots Q9BQ32 P36957 E7ESY5 B7Z5W8 Q7LDY7
Accessions AAD30181 BAA05536 EAW81199 EAW81200 EAW81201 EAW81202 P36957 AA101667 AI087261 AK289414 BAF82103 AK299505 BAH13054 AK300396 BAH13277 AK316463 BAH14834 AL538322 AU133381 BC000302 AAH00302 BC001922 AAH01922 BC002735 BM682697 BM928746 BM929953 BU623022 BX248276 CAD62604 BX248764 CAD66571 BX248774 CAD66581 CN422511 CR456727 CAG33008 CX163292 D16373 BAA03871 D17297 BAA04130 DA995927 DC338747 HQ448281 ADQ32761 L37418 AAB59629 NM_001244883 NP_001231812 NM_001933 NP_001924 NR_033814 NR_045209
Function The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Subcellular Location Mitochondrion.
Top Pathways Citrate cycle (TCA cycle), Lysine degradation, Carbon metabolism

DLST (D22B1) XP ® Rabbit mAb - 11954 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D22B1
Western blot analysis of extracts from various cell lines using DLST (D22B1) XP ® Rabbit mAb.

DLST Antibody - 5556 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using DLST Antibody.

DLST (D22G7) Rabbit mAb - 12618 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D22G7
Western blot analysis of extracts from various cell lines using DLST (D22G7) Rabbit mAb.

DLST (D-16) - sc-242583 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-DLST antibody - ab187699 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of DLST by Western Blot of Immunprecipitate.Lane 1: ab187699 at 1 µg/ml labeling DLST in Jurkat whole cell lysate immunoprecipitated using ab187699 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).Lane 2: Control IgGDetection: Chemiluminescence with exposure time of 3 seconds. 

Anti-DLST antibody - ab95946 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
IHC image of DLST staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95946, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DLST antibody - ab72790 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-DLST antibody (ab72790) at 1/500 dilution + Human liver lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-DLST antibody - ab116355 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-DLST antibody (ab116355) at 1 µg/ml + Human Placenta lysate at 10 µg

Anti-DLST antibody [9F4BD5] - ab110306 from Abcam

Prices $392.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 9F4BD5
Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

Anti-DLST antibody [EPR13318(B)] - ab177934 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13318(B)
All lanes : Anti-DLST antibody [EPR13318(B)] (ab177934) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : MCF-7 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.

Anti-DLST antibody [EPR13319(B)] - ab178692 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13319(B)
All lanes : Anti-DLST antibody [EPR13319(B)] (ab178692) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : MCF7 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.

Anti-DLST / E2 Antibody - LS-C143821 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DLST / E2 Antibody - LS-C310142 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DLST / E2 Antibody - LS-C307259 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-DLST / E2 Antibody - LS-C310143 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DLST Antibody (aa340-389) - LS-C145464 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
DLST antibody LS-C145464 Western blot of Placenta lysate. Antibody concentration 1 ug/ml.

Anti-DLST / E2 Antibody (aa50-100) - LS-C290947 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human DLST by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-DLST antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated DLST, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-DLST / E2 Antibody (clone 4D7) - LS-C197055 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D7
Western Blot analysis of DLST expression in transfected 293T cell line by DLST monoclonal antibody (M01), clone 4D7.Lane 1: DLST transfected lysate (Predicted MW: 48.8 KDa).Lane 2: Non-transfected lysate.

Anti-DLST / E2 Antibody (aa188-453) - LS-C186274 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded RT2 xenograft using DLST antibody at 1:500 dilution.

DLST Antibody - NBP1-85012 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DLST Antibody - NBP1-31715 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DLST Antibody [NBP1-31715] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.

DLST Antibody - NBP2-32204 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DLST Antibody [NBP2-32204] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-DLST antibody NBP2-32204 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

DLST Antibody - H00001743-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: DLST Antibody [H00001743-D01P] - Analysis of DLST expression in human kidney.

DLST Antibody - H00001743-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: DLST Antibody [H00001743-A01] - DLST polyclonal antibody (A01), Lot # 060803QCS1. Western Blot analysis of DLST expression in U-2 OS.

DLST Antibody - H00001743-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
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