Western Blot: Nuf2 Antibody [NBP2-32211] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32211 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Nuf2 Antibody [NBP2-32211] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32211 used for IP at 6 ug per reaction. For blotting immunoprecipitated NUF2, NBP2-32211 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.