Western Blot: LRRC8A Antibody [NBP2-32158] - LRRC8A Antibody [NBP2-32158] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-LRRC8A antibody NBP2-32158 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: LRRC8A Antibody [NBP2-32158] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-LRRC8A antibody NBP2-32158 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated LRRC8A, NBP2-32158 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.