Western Blot: HISPPD1 Antibody [NBP2-32155] - Samples: Whole cell lysate (50 ug) from K562, HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-HISPPD1 antibody NBP2-32155 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: HISPPD1 Antibody [NBP2-32155] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HISPPD1 antibody NBP2-32155 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated HISPPD1, NBP2-32155 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.