Western Blot: RRAGD Antibody [NBP2-32106] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RRAGD antibody NBP2-32106 used for IP at 6 ug per reaction. RRAGD was also immunoprecipitated by rabbit anti-RRAGD antibody BL16070. For blotting immunoprecipitated RRAGD, NBP2-32106 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Western Blot: RRAGD Antibody [NBP2-32106] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RRAGD antibody NBP2-32106 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.