RRAGD

Gene Symbol RRAGD
Entrez Gene 58528
Alt Symbol RAGD, bA11D8.2.1
Species Human
Gene Type protein-coding
Description Ras-related GTP binding D
Other Description Rag D protein|ras-related GTP-binding protein D
Swissprots A8K184 Q7L8F9 Q9NPG0 Q9NQL2
Accessions EAW48554 Q9NQL2 AF272036 AAG32663 AJ420486 AK126164 AK289799 BAF82488 AL137502 CAB70775 AL523999 BC003088 AAH03088 DB472739 JF432487 ADZ15704 XM_005248755 XP_005248812 XM_011536022 XP_011534324 NM_021244 NP_067067
Function Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. {ECO:0000269|PubMed:20381137}.
Subcellular Location Cytoplasm. Nucleus. Lysosome. Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA.
Top Pathways mTOR signaling pathway

RagD Antibody - 4470 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using RagD Antibody.

Rag D (C-15) - sc-169104 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-RRAGD antibody - ab104111 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RRAGD antibody (ab104111) at 1/100 dilution + MDA-MB435 cell lysate at 35 µg

Anti-RRAGD antibody - ab102091 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RRAGD antibody (ab102091) at 1 µg/ml + A549 cell lysate at 10 µg

Anti-RRAGD antibody - ab73090 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-RRAGD antibody (ab73090) at 1/500 dilutionLane 1 : RRAGD transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-RRAGD antibody - N-terminal - ab187679 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RRAGD antibody - N-terminal (ab187679) at 0.1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : 293T whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : TCMK-1 whole cell lysateLane 5 : NIH3T3 whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-RRAGD Antibody - LS-C131950 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RRAGD Antibody (aa171-199, FITC) - LS-C223946 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRAGD Antibody (aa171-199, PE) - LS-C223948 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRAGD Antibody (aa171-199, Azide-free, HRP) - LS-C223947 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRAGD Antibody (aa171-199, APC) - LS-C227039 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRAGD Antibody (aa171-199, AP) - LS-C227040 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRAGD Antibody (aa180-210) - LS-C324833 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRAGD Antibody (aa171-199, Biotin) - LS-C227041 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRAGD Antibody (aa287-336) - LS-C116688 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RRAGD antibody LS-C116688 Western blot of A549 cell lysate.

Anti-RRAGD Antibody (aa1-50) - LS-C290936 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RRAGD by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RRAGD antibody used for IP at 6 ug per reaction. RRAGD was also immunoprecipitated by rabbit anti-RRAGD antibody BL16070. For blotting immunoprecipitated RRAGD, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-RRAGD Antibody (aa171-199) - LS-C162765 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RRAGD Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

RRAGD Antibody - NBP1-56669 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RRAGD Antibody [NBP1-56669] - A549 cell lysate, concentration 0.2-1 ug/ml.

RRAGD Antibody - NBP2-32106 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RRAGD Antibody [NBP2-32106] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RRAGD antibody NBP2-32106 used for IP at 6 ug per reaction. RRAGD was also immunoprecipitated by rabbit anti-RRAGD antibody BL16070. For blotting immunoprecipitated RRAGD, NBP2-32106 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

RRAGD Antibody - H00058528-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: RRAGD Antibody [H00058528-B01P] - Analysis of RRAGD expression in transfected 293T cell line by RRAGD polyclonal antibody. Lane 1: RRAGD transfected lysate(44 KDa). Lane 2: Non-transfected lysate.

Anti-RRAGD (378-390) antibody produced in rabbit - SAB1102229 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human RRAGD was separated on SDS-PAGE, blotted with Anti-RRAGD (378-390) (<B>Cat. No. SAB1102229</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + RRAGD immunizing peptide (human, 378-390)

Anti-RRAGD antibody produced in rabbit - SAB2106129 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RRAGD: <B>Cat. No. SAB2106129</B>: RRAGD in A549 cell lysates with RRAGD antibody at 1.0 μg/mL.

Anti-RRAGD (31-44) antibody produced in rabbit - SAB1102228 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human RRAGD was separated on SDS-PAGE, blotted with Anti-RRAGD (31-44) (<B>Cat. No. SAB1102228</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250

Anti-RRAGD antibody produced in mouse - SAB1407825 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RRAGD: <B>Cat. No. SAB1407825</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~44 kDa using RRAGD transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

RRAGD antibody - middle region (ARP57542_P050) - ARP57542_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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