T-bet/TBX21 (D6N8B) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 13232
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D6N8B
• Applications: WB IP IHC-P ICC/IF FC ChIP
• Species Reactivities: Human
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly465 of human T-bet/TBX21 protein.
• Description: Rabbit Monoclonal
• Gene: TBX21

Product images

Western blot analysis of extracts from NK-92 and K-562 cells using T-bet/TBX21 (D6N8B) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using T-bet/TBX21 (D6N8B) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's B-cell lymphoma using T-bet/TBX21 (D6N8B) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human tonsil using T-bet/TBX21 (D6N8B) XP ® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunofluorescent analysis of NK-92 (positive, left) and K-562 (negative, right) cells using T-bet/TBX21 XP ® (D6N8B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow cytometric analysis of human PBMCs using T-bet/TBX21 (D6N8B) XP ® Rabbit mAb co-stained with either CD56, CD3 or CD19. CD56+ NK cells and a subset of CD3+ T cells are both distinctly positive for T-bet/TBX21 whereas CD19+ B cells are negative. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) #4414 was used as a secondary antibody for the T-Bet/TBX21 primary.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NK-92 cells and either 10 μl of T-bet/TBX21 (D6N8B) XP ® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CCL4 promoter primers, SimpleChIP ® Human IFN-γ Promoter Primers #13051, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.