Western Blot: SMYD3 Antibody [NBP2-20432] - IP Sample: 293T whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of SMYD3 by 2.5 ug of SMYD3 antibody 10% SDS-PAGE The immunoprecipitated SMYD3 protein was detected by SMYD3 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Immunohistochemistry-Paraffin: SMYD3 Antibody [NBP2-20432] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Chromatin Immunoprecipitation (ChIP): SMYD3 Antibody [NBP2-20432] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either control rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8.
Western Blot: SMYD3 Antibody [NBP2-20432] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.