![Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_8/20921_MAD2L2-Antibody-Western-Blot-NBP1-02798-img0003.jpg)
Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.
![Immunohistochemistry-Paraffin: MAD2L2 Antibody [NBP1-02798] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_8/20922_MAD2L2%2520Antibody-Immunohistochemistry-Paraffin-NBP1-02798-img0001.jpg)
Immunohistochemistry-Paraffin: MAD2L2 Antibody [NBP1-02798] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.