Western Blot: ZNF281 Antibody (4F7) [H00023528-M01] - Analysis of ZNF281 expression in transfected 293T cell line by ZNF281 monoclonal antibody (M01), clone 4F7.Lane 1: ZNF281 transfected lysate(96.9 KDa).Lane 2: Non-transfected lysate.
Western Blot: ZNF281 Antibody (4F7) [H00023528-M01] - Analysis of ZNF281 over-expressed 293 cell line, cotransfected with ZNF281 Validated Chimera RNAi ( Cat # H00023528-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF281 monoclonal antibody (M01), clone 4F7 (Cat # H00023528-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: ZNF281 Antibody (4F7) [H00023528-M01] - Detection limit for recombinant GST tagged ZNF281 is approximately 0.03ng/ml as a capture antibody.