ZNF281

Gene Symbol ZNF281
Entrez Gene 23528
Alt Symbol ZBP-99, ZNP-99
Species Human
Gene Type protein-coding
Description zinc finger protein 281
Other Description GC-box-binding zinc finger protein 1|ZNP-99 transcription factor|transcription factor ZBP-99|zinc finger DNA-binding protein 99
Swissprots Q5RKW5 Q9Y2X9 Q9NY92 A6NF48 B3KMX2
Accessions CAH05622 EAW91313 Q9Y2X9 AF086540 AF125158 AAD21084 AJ132591 CAB70967 AJ132592 CAB70968 AK021707 AK022916 BAG51134 AK022921 BAG51136 AK027284 BAG51299 AK094701 AK297233 BAG59716 BC016000 BC051905 AAH51905 BC060820 AAH60820 BG611294 BP216938 BQ012001 BQ013454 DB150929 NM_001281293 NP_001268222 NM_001281294 NP_001268223 NM_012482 NP_036614
Function Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G- rich box in the enhancer region of these genes. {ECO:0000250, ECO:0000269|PubMed:10448078, ECO:0000269|PubMed:12771217}.
Subcellular Location Nucleus {ECO:0000269|PubMed:10448078}.

ZNF281 (D-8) - sc-166933 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ZNF281 (D-8): sc-166933. Western blot analysis of ZNF281 expression in HeLa (A), NIH/3T3 (B) and KNRK (C) nuclear extracts.

ZNF281 (G-9) - sc-137205 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ZNF281 (G-9): sc-137205. Western blot analysis of ZNF281 expression in non-transfected: sc-117752 (A) and mouse ZNF281 transfected: sc-124785 (B) 293T whole cell lysates and KNRK nuclear extract (C).

ZNF281 (H-220) - sc-98280 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ZNF281 (H-220): sc-98280. Western blot analysis of ZNF281 expression in non-transfected: sc-117752 (A) and mouse ZNF281 transfected: sc-124785 (B) 293T whole cell lysates.

ZNF281 (K-13) - sc-103949 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ZNF281 (K-13): sc-103949. Western blot analysis of ZNF281 expression in non-transfected: sc-117752 (A) and mouse ZNF281 transfected: sc-124785 (B) 293T whole cell lysates.

ZNF281 (N-13) - sc-103950 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Zfp281 antibody - ab101318 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Zfp281 antibody (ab101318) at 1 µg/mlLane 1 : wild type mouse embryonic stem cell lysate Lane 2 : Zfp281 null mouse embryonic stem cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Zfp281 antibody - ab112047 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Zfp281 antibody (ab112047) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-Zfp281 antibody - ab89445 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Zfp281 antibody (ab89445) at 1 µg/ml + A431 cell lysate at 50 µg

Anti-Zfp281 antibody - ab154093 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Zfp281 antibody (ab154093) at 1/500 dilutionLane 1 : Non-transfected 293T cell lysateLane 2 : Zfp281 transfected 293T cell lysate.

Anti-Zfp281 antibody - ab135123 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-Zfp281 antibody (ab135123) at 1/1000 dilution + Total extracts from Human HeLa cells

Anti-Zfp281 antibody - ab75733 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Zfp281 antibody (ab75733) at 1/50 dilutionLane 1 : Non transfected 293 cell lysateLane 2 : 293 cell lysate transfected with Zfp281Lysates/proteins at 2 µg per lane.

Anti-Zfp281 antibody - ab83075 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Zfp281 antibody (ab83075) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Zfp281 antibody [148C4a] - ab53574 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 148C4a
Anti-Zfp281 antibody [148C4a] (ab53574) + immunised recombinant protein

Anti-ZNF281 / Zfp281 Antibody - LS-C76935 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-ZNF281 / Zfp281 Antibody (clone 148C4a) - LS-C184518 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 148C4a

Anti-ZNF281 / Zfp281 Antibody (clone 148C4a) - LS-C342852 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 148C4a
Western blot analysis of immunized recombinant protein, using anti-ZNF281 monoclonal antibody.

Anti-ZNF281 / Zfp281 Antibody (C-Terminus) - LS-C101724 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ZNF281 antibody LS-C101724 Western blot of HeLa lysate.

Anti-ZNF281 / Zfp281 Antibody (clone 1C9) - LS-C139200 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C9
ZNF281 monoclonal antibody (M02), clone 1C9. Western blot of ZNF281 expression in A-431.

Anti-ZNF281 / Zfp281 Antibody (aa775-825) - LS-C289819 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human ZNF281 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ZNF281 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ZNF281 was also immunoprecipitated by rabbit anti-ZNF281 antibody BL11210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Anti-ZNF281 / Zfp281 Antibody (C-Terminus) - LS-C144346 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of ZFP291 in A20 cells with ZFP291 antibody at 20 ug/ml.

ZNF281 Antibody (801706) [Unconjugated] - MAB6350 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 801706
Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ZNF281 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ZNF281 Antibody - AF6350 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and A20 mouse B cell lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), XXX µg of cytoplasmic (Cyto), and XXX µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ZNF281 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZNF281 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.

ZNF281 Antibody - NBP2-13564 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ZNF281 Antibody [NBP2-13564] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: NTERA-2 Medium

ZNF281 Antibody - NBP2-20984 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ZNF281 Antibody [NBP2-20984] - A: Non-transfected lysate. B: ZNF281 transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.

ZNF281 Antibody (4F7) - H00023528-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4F7
Western Blot: ZNF281 Antibody (4F7) [H00023528-M01] - Analysis of ZNF281 expression in transfected 293T cell line by ZNF281 monoclonal antibody (M01), clone 4F7.Lane 1: ZNF281 transfected lysate(96.9 KDa).Lane 2: Non-transfected lysate.
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