Western Blot: Importin beta Antibody [NB100-79806] - Detection of KapB1 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg) from HeLa, 293T and mouse 3T3 cells. B. Whole cell lysate (1 mg for IP, 1/4 of IP loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-KapB1 antibody BL1964 (Cat. No. NB100-79806) used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP (B). Immunoprecipitation of KapB1 was unsuccessful when using rabbit anti-KapB1 antibody BL1962 (Cat. No. NB100-79805) at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B). 25043 West