KPNB1

Gene Symbol KPNB1
Entrez Gene 3837
Alt Symbol IMB1, IPO1, IPOB, Impnb, NTF97
Species Human
Gene Type protein-coding
Description karyopherin (importin) beta 1
Other Description PTAC97|importin 1|importin 90|importin beta-1 subunit|importin subunit beta-1|importin-90|karyopherin subunit beta-1|nuclear factor p97|pore targeting complex 97 kDa subunit
Swissprots Q96J27 Q14637 Q53XN2 D3DTT3 Q14974 B7ZAV6
Accessions AAA82869 CAM84939 EAW94807 EAW94808 EAW94809 EAW94810 Q14974 AI076614 AK299145 BAH12957 AK301463 BAH13488 AK314780 BAG37316 AK316421 BAH14792 BC003572 AAH03572 BC024045 AAH24045 BC036703 AAH36703 BE046507 BQ009884 BT009797 AAP88799 DB083013 DQ892863 ABM83789 DQ896112 ABM87111 L38951 AAC41763 L39924 NM_001276453 NP_001263382 NM_002265 NP_002256
Function Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal prot
Subcellular Location Cytoplasm {ECO:0000269|PubMed:11891849}. Nucleus envelope {ECO:0000269|PubMed:11891849}.
Top Pathways RNA transport

Importin β1 Antibody - 8673 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Importin β1 Antibody.

karyopherin β1 (H-300) - sc-11367 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
karyopherin β1 (H-300): sc-11367. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing cytoplasmic staining.

karyopherin β1 (H-7) - sc-137016 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
karyopherin β1 (H-7): sc-137016. Western blot analysis of karyopherin β1 expression in BJAB (A), Jurkat (B), MDCK (C) and HeLa (D) whole cell lysates.

karyopherin β1 (N-19) - sc-1919 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
karyopherin β1 (N-19): sc-1919. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear membrane staining.

karyopherin β1 (C-19) - sc-1863 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
karyopherin β1 (C-19): sc-1863. Western blot analysis of karyopherin β1 expression in BJAB (A), Jurkat (B) and MDCK (C) whole cell lysates.

karyopherin β1 (23) - sc-136226 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
karyopherin β1 (23): sc-136226. Western blot analysis of karyopherin β1 expression in Jurkat whole cell lysate.

Anti-NTF97/Importin beta antibody - ab45938 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NTF97/Importin beta antibody (ab45938) at 2 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lysates/proteins at 15 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-NTF97/Importin beta antibody - ab45901 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NTF97/Importin beta antibody (ab45901) at 2 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lysates/proteins at 15 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-NTF97/Importin beta antibody - ab50417 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-NTF97/Importin beta antibody (ab50417) at 0.03 µg/ml + Daudi cell lysate (35µg protein in RIPA buffer).

Anti-NTF97/Importin beta antibody - N-terminal - ab135614 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NTF97/Importin beta antibody - N-terminal (ab135614) at 1/50 dilution + Y79 cell line lysates at 35 µg

Anti-KPNB1 / Importin Beta Antibody - LS-C16995 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-KPNB1 / Importin Beta Antibody - LS-C71266 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-KPNB1 / Importin Beta Antibody (clone 2501C3a) - LS-C184405 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2501C3a

Anti-KPNB1 / Importin Beta Antibody - LS-C73759 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-KPNB1 / Importin Beta Antibody (C-Terminus) - LS-C287366 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KPNB1 / Importin Beta Antibody (aa144-193) - LS-C145499 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
KPNB1 antibody LS-C145499 Western blot of ACHN Cell lysate. Antibody concentration 1 ug/ml.

Anti-KPNB1 / Importin Beta Antibody (clone 2501C3a) - LS-C342451 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2501C3a
Western blot analysis of immunized recombinant protein, using anti-KPNB1 monoclonal antibody.

Anti-KPNB1 / Importin Beta Antibody (aa826-876) - LS-C288584 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse KPNB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-KPNB1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Anti-KPNB1 / Importin Beta Antibody (aa851-863) IHC-plus™ - LS-B2533 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-KPNB1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2533 concentration 5 ug/ml.

Anti-KPNB1 / Importin Beta Antibody (aa190-216) IHC-plus™ - LS-B11745 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-KPNB1 / Importin Beta Antibody (aa1-50) - LS-C287365 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Importin beta/KPNB1 Antibody (3E9) - NB120-2811 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E9
Western Blot: Importin beta Antibody (3E9) [NB120-2811] - U251 (lane 1), HepG2 (lane 2) and C6 (lane 3)

Importin beta/KPNB1 Antibody (845208) - MAB8209 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 845208
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Importin beta /KPNB1 Monoclonal Antibody (Catalog # MAB8209) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Importin beta /KPNB1 at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Importin beta/KPNB1 Antibody - NBP1-87712 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Importin beta/KPNB1 Antibody [NBP1-87712] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Importin beta/KPNB1 Antibody - NB100-79806 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Importin beta Antibody [NB100-79806] - Detection of KapB1 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg) from HeLa, 293T and mouse 3T3 cells. B. Whole cell lysate (1 mg for IP, 1/4 of IP loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-KapB1 antibody BL1964 (Cat. No. NB100-79806) used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP (B). Immunoprecipitation of KapB1 was unsuccessful when using rabbit anti-KapB1 antibody BL1962 (Cat. No. NB100-79805) at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B). 25043 West
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