![Western Blot: SEC22B Antibody [NBP2-37701] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-SEC22B antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/29690_SEC22B-Antibody-Western-Blot-NBP2-37701-img0001.jpg)
Western Blot: SEC22B Antibody [NBP2-37701] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-SEC22B antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
![Immunoprecipitation: SEC22B Antibody [NBP2-37701] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-SEC22B antibody used for IP at 6 ug per reaction. SEC22B was also immunoprecipitated by rabbit anti-SEC22B antibody. For blotting immunoprecipitated SEC22B, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/29691_SEC22B-Antibody-Immunoprecipitation-NBP2-37701-img0002.jpg)
Immunoprecipitation: SEC22B Antibody [NBP2-37701] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-SEC22B antibody used for IP at 6 ug per reaction. SEC22B was also immunoprecipitated by rabbit anti-SEC22B antibody. For blotting immunoprecipitated SEC22B, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.