Western Blot: PTPN23 Antibody [NBP2-36549] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HD-PTP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PTPN23 Antibody [NBP2-36549] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. For blotting immunoprecipitated HD-PTP, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.