Western Blot: FLVCR Antibody [NBP2-36564] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-FLVCR1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: FLVCR Antibody [NBP2-36564] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. For blotting immunoprecipitated FLVCR1, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.