![Western Blot: VCPIP1 Antibody [NBP1-49940] - Detection of Human and Mouse VCIP135 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-VCIP135 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. VCIP135 was also immunoprecipitated by rabbit anti-VCIP135 antibody which recognizes an upstream epitope. For blotting immunoprecipitated VCIP135 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 second (B).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/6611_VCPIP1%2520Antibody-Western%2520Blot-NBP1-49940-img0001.jpg)
Western Blot: VCPIP1 Antibody [NBP1-49940] - Detection of Human and Mouse VCIP135 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-VCIP135 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. VCIP135 was also immunoprecipitated by rabbit anti-VCIP135 antibody which recognizes an upstream epitope. For blotting immunoprecipitated VCIP135 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 second (B).