VCPIP1

Gene Symbol VCPIP1
Entrez Gene 80124
Alt Symbol DUBA3, VCIP135
Species Human
Gene Type protein-coding
Description valosin containing protein (p97)/p47 complex interacting protein 1
Other Description VCP/p47 complex-interacting 135-kDa protein|deubiquitinating protein VCIP135|valosin-containing protein (p97)/p47 complex-interacting protein p135|valosin-containing protein p97/p47 complex-interacting protein 1|valosin-containing protein p97/p47 complex-interacting protein p135
Swissprots Q8N3A9 Q86W01 Q96JH7 Q9H5R8 Q504T4 Q86T93
Accessions EAW86916 Q96JH7 AB058753 BAB47479 AK026785 BAB15552 AK225580 AK297345 BAG59796 AL832606 CAD89944 AL834476 CAD39135 AM392649 CAL37527 AM393766 CAL38641 BC012498 BC049379 AAH49379 BC062426 BC094799 AAH94799 BC118917 BM980302 CR936659 NM_025054 NP_079330
Function Acts as a deubiquitinating enzyme. Necessary for VCP- mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity). Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains. {ECO:0000250, ECO:0000269|PubMed:23827681}.
Subcellular Location Endoplasmic reticulum {ECO:0000250}. Golgi apparatus, Golgi stack {ECO:0000250}. Note=Associated with Golgi stacks and endoplasmic reticulum. {ECO:0000250}.

VCPIP1 (C-15) - sc-98197 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
VCPIP1 (C-15): sc-98197. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells.

VCPIP1 (N-12) - sc-98199 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-VCIP 135 antibody - ab97812 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-VCIP 135 antibody (ab97812) at 1/5000 dilution + H1299 whole cells lysate at 30 µg

Anti-VCIP 135 antibody - ab123981 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-VCIP 135 antibody (ab123981) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

Anti-VCPIP1 Antibody (aa124-151, APC) - LS-C242561 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VCPIP1 Antibody (aa124-151, Biotin) - LS-C242562 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VCPIP1 Antibody (aa124-151, AP) - LS-C242564 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VCPIP1 Antibody (aa124-151, FITC) - LS-C242565 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VCPIP1 Antibody (aa124-151, PE) - LS-C242566 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VCPIP1 Antibody (aa124-151, Azide-free, HRP) - LS-C247217 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VCPIP1 Antibody (aa1172-1222) - LS-C289642 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-VCPIP1 Antibody (aa123-151) - LS-C326259 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VCPIP1 Antibody (aa124-151) - LS-C160505 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
VCIP1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The VCIP1 antibody detected the VCIP1 protein (arrow).

Anti-VCPIP1 Antibody (aa1100-1150) - LS-C289641 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human VCIP135 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-VCIP135 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

VCIP135/VCPIP1 Antibody - NBP1-32663 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VCIP135/VCPIP1 Antibody [NBP1-32663] -  A: H1299.

VCIP135/VCPIP1 Antibody - NBP1-49940 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VCPIP1 Antibody [NBP1-49940] - Detection of Human and Mouse VCIP135 by Western Blot (h&m) and Immunoprecipitation (h).  Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-VCIP135 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  VCIP135 was also immunoprecipitated by rabbit anti-VCIP135 antibody which recognizes an upstream epitope.  For blotting immunoprecipitated VCIP135 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 second (B).

VCIP135/VCPIP1 Antibody - NBP1-49939 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VCIP135/VCPIP1 Antibody [NBP1-49939] - Detection of Human VCIP135/VCPIP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-VCIP135 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  VCIP135 was also immunoprecipitated by rabbit anti-VCIP135 antibody which recognizes a downstream epitope.  For blotting immunoprecipitated VCIP135 was used.   Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

VCIP135/VCPIP1 Antibody - H00080124-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Anti-VCPIP1 (C-terminal) antibody produced in rabbit - SAB2700048 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-VCPIP1 (C-terminal): <B>Cat. No. SAB2700048</B>: Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using VCPIP1 antibody at 1:500 dilution.

Anti-VCPIP1 antibody produced in rabbit - HPA023932 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-VCPIP1:<B>Cat. No. HPA023932</B>: Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

ANTI-VCIP1 (N-TERM) antibody produced in rabbit - SAB1302315 from Sigma-Aldrich

Prices $326.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

VCIP1-Antibody-N-term - AP16265a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
VCIP1 Antibody (N-term) (Cat. #AP16265a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the VCIP1 antibody detected the VCIP1 protein (arrow).

VCIP1 Antibody - N-terminal region (OAAB11670) - OAAB11670 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

VCPIP1 antibody - orb74273 from Biorbyt

Prices $723.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal
Western Blot analysis of A:H1299 whole cell lysate using VCPIP1 antibody

VCPIP1 antibody - 70R-21246 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
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