Western Blot: Apc1 Antibody [NB100-86985] - Detection of Human and Mouse APC1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-APC1 antibody NB100-86985 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. APC1 was also immunoprecipitated by another rabbit anti-APC1 antibody which recognizes an upstream epitope.