Western Blot: NSUN2 Antibody [NBP2-22264] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: NSUN2 Antibody [NBP2-22264] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22264 used for IP at 6 ug/mg lysate. Misu was also immunoprecipitated by rabbit anti-Misu antibody. For blotting immunoprecipitated Misu, NBP2-22264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.