NSUN2

Gene Symbol NSUN2
Entrez Gene 54888
Alt Symbol MISU, MRT5, SAKI, TRM4
Species Human
Gene Type protein-coding
Description NOP2/Sun RNA methyltransferase family, member 2
Other Description 5-methycytoisine methyltransferase|Myc-induced SUN-domain-containing protein|NOL1/NOP2/Sun domain family, member 2|NOP2/Sun domain family, member 2|myc-induced SUN domain-containing protein|substrate of AIM1/Aurora kinase B|tRNA (cytosine(34)-C(5))-methyltransferase|tRNA (cytosine-5-)-methyltransferase NSUN2|tRNA methyltransferase 4 homolog
Swissprots Q08J23 G3V1R4 B4DQW2 B3KP09 Q9NXD9 Q9BVN4 B2RNR4 A8K529 Q9H858
Accessions EAX08105 EAX08106 EAX08107 Q08J23 AB255451 BAF34150 AK000310 BAA91075 AK023994 BAB14762 AK055456 BAG51521 AK291144 BAF83833 AK298980 BAG61074 BC001041 AAH01041 BC137083 AAI37084 BF000644 BG387061 BQ689094 NM_001193455 NP_001180384 NM_017755 NP_060225 NR_037947
Function RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5- methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity. {ECO:0000269|PubMed:17071714, ECO:0000269|PubMed:19596847, ECO:0000269|PubMed:22995836}.
Subcellular Location Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle. Note=Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA.
Tissue Specificity Expressed in adult and fetal brain and in lymphoblastoid cells. {ECO:0000269|PubMed:22541559}.

NSUN2 (H-224) - sc-366094 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NSUN2 (H-224): sc-366094. Western blot analysis of NSUN2 expression in HeLa (A), MCF7 (B), SH-SY5Y (C), RT-4 (D), Jurkat (E) and IMR-32 (F) whole cell lysates.

NSUN2 (I-16) - sc-83444 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NSUN2 (I-16): sc-83444. Western blot analysis of NSUN2 expression in HeLa (A), COLO 320DM (B), Hep G2 (C), MCF7 (D) and U-698-M (E) whole cell lysates.

NSUN2 (N-19) - sc-83445 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NSUN2 (N-19): sc-83445. Western blot analysis of NSUN2 expression in HeLa (A), Hep G2 (B), MCF7 (C), U-251-MG (D) and U-698-M (E) whole cell lysates.

Anti-NSUN2 antibody - ab157093 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NSUN2 antibody (ab157093) at 0.1 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-NSUN2 antibody - ab107262 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NSUN2 antibody (ab107262) at 1/100 dilution + Mouse spleen tissue lysates at 35 µg

Anti-NSUN2 antibody - ab128243 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NSUN2 antibody - ab75493 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NSUN2 antibody (ab75493) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-NSUN2 antibody - ab188543 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NSUN2 Antibody (aa614-663) - LS-C81300 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-NSUN2 Antibody (aa423-451) - LS-C163023 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
NSUN2 Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The NSUN2 antibody detected the NSUN2 protein (arrow).

Anti-NSUN2 Antibody - LS-C332574 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using NSUN2 antibody.

Anti-NSUN2 Antibody (aa425-475) - LS-C290558 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Misu by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Misu antibody used for IP at 6 ug/mg lysate. Misu was also immunoprecipitated by rabbit anti-Misu antibody BL14092. For blotting immunoprecipitated Misu, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-NSUN2 Antibody (Internal) - LS-C153600 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NSUN2 Antibody (aa423-451, AP) - LS-C238179 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSUN2 Antibody (aa423-451, APC) - LS-C238180 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSUN2 Antibody (aa423-451, Biotin) - LS-C238181 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSUN2 Antibody (aa423-451, FITC) - LS-C238182 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSUN2 Antibody (aa423-451, PE) - LS-C238183 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSUN2 Antibody (aa423-451, Azide-free, HRP) - LS-C248955 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSUN2 Antibody (aa426-455) - LS-C323464 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

NSUN2 Antibody - NBP1-86878 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NSUN2 Antibody - NBP1-53052 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NSUN2 Antibody [NBP1-53052] - Hela cell lysate, concentration 0.2-1 ug/ml.

NSUN2 Antibody - NBP1-82189 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NSUN2 Antibody - NBP2-22264 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: NSUN2 Antibody [NBP2-22264] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-NSUN2 antibody produced in rabbit - AV48811 from Sigma-Aldrich

Prices $347.50
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: HeLa (<B>Cat. No. AV48811</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1 μg/mL in cell type HeLa
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