Western Blot: PAPD1 Antibody [NBP2-22258] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PAPD1 Antibody [NBP2-22258] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: NBP2-22258 used for IP at 6 ug/mg lysate. PAPD1 was also immunoprecipitated by another rabbit anti-PAPD1 antibody. For blotting immunoprecipitated PAPD1, NBP2-22258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.