Western Blot: EDF1 Antibody [NBP2-22327] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22327 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: EDF1 Antibody [NBP2-22327] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- EDF-1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: EDF1 Antibody [NBP2-22327] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22327 used for IP at 6 ug/mg lysate. EDF-1 was also immunoprecipitated by rabbit anti-EDF-1 antibody. For blotting immunoprecipitated EDF-1, NBP2-22327 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.