Western Blot: ASCC3L1 Antibody [NBP1-79037] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody NBP1-79036 which recognizes an upstream epitope.
Immunohistochemistry: ASCC3L1 Antibody [NBP1-79037] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SNRNP200 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).