SNRNP200

Gene Symbol SNRNP200
Entrez Gene 23020
Alt Symbol ASCC3L1, BRR2, HELIC2, RP33, U5-200KD
Species Human
Gene Type protein-coding
Description small nuclear ribonucleoprotein 200kDa (U5)
Other Description BRR2 homolog|U5 small nuclear ribonucleoprotein 200 kDa helicase|U5 snRNP-specific 200 kDa protein|activating signal cointegrator 1 complex subunit 3-like 1
Swissprots Q9H7S0 O94884 Q6PX59 Q6NZY0 Q96IF2 O75643 Q8NBE6
Accessions CAA94089 EAW71372 EAW71373 O75643 AB018331 BAA34508 AF085356 AAD40191 AF119874 AAF69628 AJ844618 CAH59741 AK021418 AK021583 AK023840 BAB14698 AK024391 BAB14906 AK026662 AK090671 BAC03499 AK303465 BAG64507 AK303663 BAG64662 AL831994 AW190145 AY572488 AAS78571 BC001417 AAH01417 BC007577 AAH07577 BC065924 AAH65924 BC112891 AAI12892 BC131784 AAI31785 BP873066 DA109508 DB067751 NM_014014 NP_054733
Function RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome. {ECO:0000269|PubMed:16723661, ECO:0000269|PubMed:23045696, ECO:0000269|PubMed:8670905, ECO:0000269|PubMed:9539711}.
Subcellular Location Nucleus {ECO:0000269|PubMed:9539711}.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:19878916}.
Top Pathways Spliceosome

HELIC2 (G-9) - sc-393170 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HELIC2 (G-9): sc-393170. Western blot analysis of HELIC2 expression in Jurkat (A), BJAB (B) and HeLa (C) nuclear extracts and RAW 264.7 whole cell lysate (D).

HELIC2 (B-11) - sc-393517 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HELIC2 (B-11): sc-393517. Western blot analysis of HELIC2 expression in Jurkat (A), BJAB (B) and HeLa (C) nuclear extracts and RAW 264.7 whole cell lysate (D).

HELIC2 (H-300) - sc-367658 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HELIC2 (H-300): sc-367658. Western blot analysis of HELIC2 expression in MCF7 (A), CCRF-CEM (B), PC-3 (C) and Hs 181 Tes (D) whole cell lysates.

HELIC2 (N-20) - sc-68563 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HELIC2 (N-20): sc-68563. Western blot analysis of HELIC2 expression in HeLa (A), Jurkat (B), BJAB (C) and RAW 264.7 (D) nuclear extracts.

Anti-SNRNP200 antibody - ab176715 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
SNRNP200 was immunoprecipitated from 1mg HeLa whole cell lysate using ab176715 at 6 μg/mg lysate (Lane 2), a rabbit anti-SNRNP200 antibody recognizing an upstream epitope (Lane 1) or control IgG (Lane 3). 20% of the Immunoprecipitate was loaded per lane and then immunobotted using ab176715 at 1.0 μg/ml.Detection:Chemiluminescence with exposure time of 10 seconds.

Anti-SNRNP200 antibody - ab118713 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-SNRNP200 antibody (ab118713) at 1 µg/ml + COLO205 lysate at 10 µgSecondaryGoat anti-Rabbit IgG-HRP

Anti-SNRNP200 antibody - C-terminal - ab189616 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-SNRNP200 antibody - C-terminal (ab189616) at 1/1000 dilutionLane 1 : Mouse brain total cell extractLane 2 : Human Jurkat total cell extract

Anti-SNRNP200 Antibody (aa1-50) - LS-C290126 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SNRNP200 Antibody (aa400-450) - LS-C290129 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SNRNP200 Antibody - LS-C334497 from LifeSpan Bioscience

Prices $315.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using SNRNP200 antibody.

Anti-SNRNP200 Antibody (aa212-261) - LS-C145856 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SNRNP200 antibody LS-C145856 Western blot of COL0205 Cell lysate. Antibody concentration 1 ug/ml.

ASCC3L1 Antibody - NBP1-90086 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ASCC3L1 Antibody - NBP1-79037 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ASCC3L1 Antibody [NBP1-79037] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody NBP1-79036 which recognizes an upstream epitope.

ASCC3L1 Antibody - NBP1-79036 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ASCC3L1 Antibody [NBP1-79036] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody NBP1-79037, which recognizes a downstream epitope.

Anti-SNRNP200 antibody produced in rabbit - HPA029321 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-SNRNP200: <B>Cat. No. HPA029321</B>: Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Anti-SNRNP200 antibody produced in rabbit - SAB2106387 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SNRNP200: <B>Cat. No. SAB2106387</B>: Western blot analysis of SNRNP200 in COLO205 with SNRNP200 (small nuclear ribonucleoprotein 200kDa, U5) antibody, against the N terminal of SNRNP200 (50 μg) antibody at 1.0 μg/mL.

SNRNP200 antibody - N-terminal region (ARP36414_P050) - ARP36414_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SNRNP200 Polyclonal Antibody - A6063 from ABclonal

Prices $130.00, $220.00, $380.00
Sizes 50 µl, 100 µl, 200 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using SNRNP200 antibody.

SNRNP200 Antibody - abx004666 from Abbexa

Host Rabbit
Clonality Polyclonal

ASCC3L1 antibody - 23875-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
HeLa cells were subjected to SDS PAGE followed by western blot with 23875-1-AP(SNRNP200 antibody) at dilution of 1:1000

Anti-SNRNP200, Polyclonal Antibody - 25-A6063 from ARP American Research Products

Prices $279.00, $549.00
Sizes 50 µg, 200 µg
Host Rabbit
Clonality Polyclonal

Anti-SNRNP200 antibody - STJ27857 from St John's Laboratory Ltd

Host Rabbit
Clonality Polyclonal

SNRNP200 antibody - 23875-1-AP from Proteintech Group

Prices $279.00
Sizes con: 32 μg/150μl
Host Rabbit
Clonality Polyclonal
HeLa cells were subjected to SDS PAGE followed by western blot with 23875-1-AP(SNRNP200 antibody) at dilution of 1:1000

SNRNP200 - A6063 from NeoScientific

Prices $380.00, $620.00
Sizes 100 µl, 200 µl
Host Rabbit
Clonality Polyclonal

SNRNP200 Antibody - GWB-MM385G from Genway Biotech

Prices $368.00
Sizes 50 µg
Host Rabbit
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