Western blot analysis of extracts from 293T cells, untreated (-) or λ-phosphatase treated (+), and XP2Y0 cells, using Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb (top), Topoisomerase IIα (D10G9) XP ® Rabbit mAb #12286 (middle) or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (bottom).
Confocal immunofluorescent analysis of 293T cells, untreated (left) or phosphatase-treated (center), and XP2YO cells (right) using Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).