TOP2A

Gene Symbol TOP2A
Entrez Gene 7153
Alt Symbol TOP2, TP2A
Species Human
Gene Type protein-coding
Description topoisomerase (DNA) II alpha 170kDa
Other Description DNA gyrase|DNA topoisomerase (ATP-hydrolyzing)|DNA topoisomerase 2-alpha|DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme
Swissprots Q9HB24 P11388 Q9UQP9 Q9HB25 Q71UQ5 Q71UN1 Q9HB26 B2RTS1 Q9UP44
Accessions AAC16736 AAC23518 AAC77388 ABA01496 CAA09762 EAW60661 EAW60662 EAW60663 P11388 AF285157 AAG13403 AF285158 AAG13404 AF285159 AAG13405 AK024080 BC013429 BC062677 BC140791 AAI40792 DB069825 J04088 AAA61209 L47276 L47277 XM_005257632 XP_005257689 XM_011525165 XP_011523467 NM_001067 NP_001058
Function Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity). {ECO:0000250|UniProtKB:Q01320, ECO:0000269|PubMed:18790802, ECO:0000269|PubMed:22013166, ECO:0000269|PubMed:22323612}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:22013166}. Nucleus, nucleoplasm {ECO:0000269|PubMed:22013166}. Note=Generally located in the nucleoplasm.

Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb - 13072 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4F5
Western blot analysis of extracts from 293T cells, untreated (-) or λ-phosphatase treated (+), and XP2Y0 cells, using Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb (top), Topoisomerase IIα (D10G9) XP ® Rabbit mAb #12286 (middle) or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (bottom).

Topoisomerase IIα (D10G9) XP ® Rabbit mAb - 12286 from Cell Signaling Technology

Prices $109.00, $282.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10G9
Western blot analysis of extracts from various cell lines using Topoisomerase IIα (D10G9) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Topoisomerase IIα Antibody - 4733 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MDA-MB-468, MDA-MB-231 and HeLa cells, using Topoisomerase IIalpha Antibody.

Phospho-Topoisomerase IIalpha (Ser1106) Antibody - 2418 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and K562 cells, using Phospho-Topoisomerase IIalpha (Ser1106) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) (right) or without (-) (left) calf intestinal phosphatase (CIP) after Western transfer.

Topoisomerase IIalpha/beta Antibody - 4734 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and Neuro2A cells, using Topoisomerase IIalpha/beta Antibody.

Topo IIα (B-6) - sc-365918 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo IIα (B-6): sc-365918. Western blot analysis of Topo IIα expression in K-562 nuclear extract.

Topo IIα (C-15) - sc-5346 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Topo IIα (C-15): sc-5346. Western blot analysis of Topo IIα expression in HeLa (A), K-562 (B) and Jurkat (C) nuclear extracts.

Topo IIα (E-10) - sc-365799 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo IIα (E-10): sc-365799. Western blot analysis of Topo IIα expression in Jurkat nuclear extract.

Topo IIα (E-11) - sc-365917 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo IIα (E-11): sc-365917. Western blot analysis of Topo IIα expression in K-562 nuclear extract.

Topo IIα (G-9) - sc-166232 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo IIα (G-9): sc-166232. Western blot analysis of Topo IIα expression in K-562 (A) and Jurkat (B) nuclear extracts.

Topo IIα (G-6) - sc-166934 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo IIα (G-6): sc-166934. Western blot analysis of Topo IIα expression in Jurkat nuclear extract.

Topo IIα (F-12) - sc-365916 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo IIα (F-12): sc-365916. Western blot analysis of Topo IIα expression in K-562 nuclear extract.

Topo IIα (H-231) - sc-13058 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Topo IIα (H-231): sc-13058. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.

Topo IIα (K-19) - sc-5347 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Topo IIα (K-19): sc-5347. Western blot analysis of Topo IIα expression in HeLa (A), K-562 (B) and Jurkat (C) nuclear extracts.

Topo IIα (A-8) - sc-165986 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo IIα (A-8): sc-165986. Western blot analysis of Topo IIα expression in K-562 (A) and Jurkat (B) nuclear extracts.

Topo IIα (S-20) - sc-5348 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Topo IIα (S-20): sc-5348. Western blot analysis of Topo IIα expression in HeLa (A) and K-562 (B) nuclear extracts.

Topo IIα (3F6) - sc-56803 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl supernatant
Host Mouse
Clonality Monoclonal
Topo IIα (3F6): sc-56803. Western blot analysis of Topo IIα expression in K-562 (A), Jurkat (B) and C32 (C) nuclear extracts.

p-Topo IIα (Thr 1343) - sc-16742 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Topo IIα (Thr 1343): sc-16742. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovary tumor showing nuclear localization.

Anti-Topoisomerase II alpha (phospho S1106) antibody - ab138667 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Topoisomerase II alpha (phospho S1106) antibody (ab138667) at 1/500 dilution + Jurkat cell lysate (from cells treated with 1µM paclitaxel for 24h) at 30 µg

Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] - ab75765 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2722
All lanes : Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765) at 1/500 dilutionLane 1 : HeLa cell lysates, untreatedLane 2 : HeLa cell lysates, treated with APLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-Topoisomerase II alpha (phospho S1525) antibody - ab138683 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Topoisomerase II alpha (phospho S1525) antibody (ab138683) at 1/500 dilution + RAW 264.7 cell extracts, treated with TNF (20ng/ml, 5 mins) at 30 µg

Anti-Topoisomerase II alpha (phospho T1343) antibody - ab138657 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Topoisomerase II alpha (phospho T1343) antibody (ab138657) at 1/500 dilution + HepG2 extracts (from cells treated with Ca2+ at 40nM for 30 minutes) at 30 µg

Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - ab52853 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1054Y
All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilutionLane 1 : HeLa cell lysate treated with APLane 2 : HeLa cell lysate untreatedLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-Topoisomerase II alpha antibody - ab12318 from Abcam

Prices $390.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryangeal squamous cell carcinoma labelling Topoisomerase II alpha with ab12318 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Topoisomerase II alpha antibody - ab74715 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Topoisomerase II alpha antibody (ab74715) at 1/500 dilutionLane 1 : extracts from HT-29 cellsLane 2 : extracts from HT-29 cells with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.
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