Western Blot: SLC1A5 Antibody [NBP2-32239] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SLC1A5 antibody NBP2-32239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: SLC1A5 Antibody [NBP2-32239] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-SLC1A5 antibody NBP2-32239 used for IP at 6 ug per reaction. For blotting immunoprecipitated SLC1A5, NBP2-32239 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.