Western Blot: Ribophorin II Antibody [NBP2-32178] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Ribophorin II antibody NBP2-32178 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: Ribophorin II Antibody [NBP2-32178] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- RPN2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunohistochemistry: Ribophorin II Antibody [NBP2-32178] - Sample: FFPE section of human mammary glands. Antibody: Affinity purified rabbit anti- RPN2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: Ribophorin II Antibody [NBP2-32178] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Ribophorin II antibody NBP2-32178 used for IP at 6 ug per reaction. Ribophorin II was also immunoprecipitated by rabbit anti-Ribophorin II antibody BL15802. For blotting immunoprecipitated Ribophorin II, NBP2-32178 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.