Western blot analysis of extracts from various cell lines using CD71 (D7G9X) XP ® Rabbit mAb.
Immunoprecipitation of CD71 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or CD71 (D7G9X) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CD71 (D7G9X) XP ® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Brefeldin A #9972 (5 μg/ml, 15 min; right), using CD71 (D7G9X) XP ® Rabbit mAb (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or anti-human CD3 (10ug/ml, 72 hours, 37C) and anti-human CD28 (5ug/ml, 72 hours, 37C) treated using CD71 (D7G9X) XP(R) Rabbit mAb and co-stained with a CD3 antibody. Anti-rabbit IgG (H+L), F(ab)'2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody.