Western blot analysis of Cox-2 expression in uninduced (A,C) and LPS + PMA-treated (B,D) RAW 264.7 whole cell lysates. Antibodies tested include Cox-2 (C-20): sc-1745 (A,B) and Cox-2 (M-19): sc-1747 (C,D).
Cox-2 (C-20): sc-1745. Immunofluorescence staining of methanol-fixed LPS + PMA-treated (A) and untreated control (B) RAW 264.7 cells.
Cox-2 (C-20): sc-1745. Western blot analysis of Cox-2 expression in non-transfected: sc-110760 (A) and human Cox-2 transfected: sc-113099 (B) 293 whole cell lysates.
Cox-2 (C-20): sc-1745. Western blot analysis of Cox-2 expression in A549 whole cell lysate.
Cox-2 (C-20): sc-1745. Western blot analysis of Cox-2 expression in untreated (A) and DuP-697 (sc-200680) treated (B) A549 whole cell lysates. Note down regulation of Cox-2 expression in lane B.
Cox-2 (D-12): sc-1745. Western blot analysis of Cox-2 expression in untreated (A) and DMNQ (sc-202586) treated (B) RAW 264.7 whole cell lysates. Note upregulation of Cox-2 expression in lane B.
Cox-2 (C-20) PE: sc-1745 PE. Intracellular FCM analysis of fixed and permeabilized HUV-EC-C cells. Black line histogram represents the isotype control, normal goat IgG: sc-3992.